This repository belongs to the observation experiments for tracking lineages of Seminavis robusta. The connected work reconstructed the lineages and obtained individuals' timing of division and motility. Cells were entrained for 5 days prior under white light with 25 µmol phot / s in a 12:12 hour rhythm. The images were recorded with a modified Leica microscope (DMIL) and 770 nm light source and a band pass filter. Entrainment continued for the time of recording.
These directories contain the full-scale raw data from the experiments. Images were captured at 10-second intervals using IC Capture (The Imaging Source).
Directory names include the starting date, experiment type, exposure time, gain (two decimal places), gamma (two decimal places), and sharpness parameter.
Image filenames end with a timestamp. Preceding the timestamp (in order) are: the researcher’s name (Jonas Ziebarth), magnification (4x), lighting (IR-LED), observed species (Seminavis robusta), and experiment type. In the raw images, 129 pixels equate to 100 µm.
Use: The data is processed by the Diatom Tracker program (part of the connected work). In the first file selection dialogue, select the directories for raw data and saving. The program subsequently reads the files, reduces image resolution, segments content, and finally exports results to video and CSV files.

This repository item contains a dummy file only. The actual dataset is too large to be downloaded directly via the repository and is therefore stored in a separate location. It is approx. 8 TB in size.
To obtain the full dataset, please use the repository’s request function (click on the lock symbol next to the file). After submitting a request, data transfer will be arranged individually with the requester (e.g. via secure download, physical media, or institutional transfer), depending on size and access requirements.

The recording of data started after the beginning of the dark phase. The offsets for the respective data sets are:
20240121_... +13 frames / 130 s
20240217_... +384 frames / 3840 s
20240301_... +21 frames / 210 s

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